SUMMARY
More than 15 years of combined teaching and academy experience including 10 years of solid hands-on experience of continuous work on molecular cloning and genetic diagnosis.Solid scientific track record.? A strong interest in molecular biology, cancer research and immunology.

EDUCATION
Department of Molecular Biology and Biochemistry, 4th Military Medical University
Xi’an,P. R. China, Jun. 1996
Ph.D., Molecular Biology

Fourth Military Medical University
Xi’an, P. R. China, Jul. 1990
M.D., Biochemistry

PROFESSIONAL EXPERIENCE
Jan.2007-Current
Zhejiang University-Adinovo Center for Genetic and Genomic Medicine,
Hangzhou, P.R.China
Research: Pharmacogenomics and Personalized Medicine

May.2006-Dec.2006? ?Senior Scientist & Professor
National Engineering Research Center for Miniaturized Detection System
Shaanxi Lifegen Co. LTD
Northwest University, Xi’an China,
Major work: Blood substitutes.

Apr.2005-May.2006? General Manager
Hailiang Biotech CO. LTD
Haikou, P.R. China.

R&D summary: new biomedicines, including the IFNa-2a/2b, G-CSF, GM-CSF, thymosin a1, GLP-1 derivates, astaxanthin, ademetionine.?

Apr. 2002-Mar. 2005? Vice director, associated professor.
The institute of gene diagnosis
Fourth Military medical University,
Xi’an P.R. China

Research area: new technology and methods in gene diagnosis.
Research summary: (1) Techniques in SNP analysis and detection (i.g. TDI-FP, Pyrosequence); (2) Genetic analysis and risk prediction of inherited disease. (i.g. Fragile X syndrome, diabetes mellitus, coronary artery disease); (3) Gene diagnosis and resistance detection of pathogenic microorganism ( i.g. HBV, HPV, Mycobacterium tuberculosis, helicobacter pylori).

Aug. 1999-Apr.2002 Post Doc.
Department of Radiation Oncology
New England Medical Center Hospital
Tufts University Medical School
Boston, MA, U.S. A

Research area: The role of tumor suppressor gene BRCA2 in breast cancer.
Research summary: (1)Selection of the BRCA2 interactive protein using yeast two hybrid system.(2) In vitro and in vivo binding assay of the interactive proteins. (3)The biological function of the interactive proteins and their roles in breast cancer抯 development and prevention.? The major contribution is that I found a novel centrosome protein named as 揷entribin”, which plays a role in the centrosome amplification and duplication.?
Techniques used: (1) Yeast two hybrid system. (2)DNA cloning, mapping and sequencing structure analysis of nuclear acid and protein with computer. (3) IP, co-IP and Western blot techniques. (3) Cell culture, transfection, luciferase assay and immunochemistry assays.

Oct. 1998-Jun.1999 Post doc.
Agriculture Research Service, Forage and Range Research Laboratory,
Utah State University
Logan, UT, USA

Research summary: selection of the molecular markers in grass seeds using RAPD (Random Amplified Polymorphic DNA), and AFLP( Amplified Fragment Length Polymorphic analysis).

Jun.1996-Nov. 1998 Lecture,
Department of Biochemistry and Molecular Biology,
Fourth Military Medical University.
Xi’an, P.R. China

Research summary: (1) Construction and expression of anti-CD3/anti-melanoma bi-specific single chain antibody. (granted by Chinese National Natural Science Foundation). (2) Construction and expression of the anti-gama-seminoprotein ScFv/carboxypeptidase fusion (granted by Military Sanitation and Science Foundation). (3)? Signal transduction.? Clone and expression PH domains; selection and identification of the itk/PH domain interactive proteins with yeast two-hybrid system. (granted by Chinese National Natural Science Foundation).

Techniques used: DNA cloning, mapping and sequencing, primer design, structure analysis of nuclear acid and protein with computer, Southern, Northern and Western blot techniques, construction of expression vector and target gene expression, RT-PCR and PCR technique,? phage display, SDS-PAGE, HPLC, FPLC and other techniques related with preparation and purification of recombinant protein, Cell culture, immunochemistry assays.


Jul.1993-Jun. 1996 Ph.D. candidate
Department of Molecular Biology and Biochemistry,
Fourth Military Medical University
Xi’an, P.R. China

Research summary: Construction, expression and Identification of human anti-CD3 single Chain antibody.
Thesis title: Construction, Expression and Activity of the Anti- Human CD3 Single Chain Fv
Techniques used: PCR, RT-PCR, molecular cloning, library construction and screening, phage display, expression, separation and purification of recombinant proteins, Western blot, ELISA, flow cytometry, generation and testing of monoclonal antibodies.

?Jul.1990-Jul.1993 Physician
Department of Gastroenterology, Xi-Jing hospital

Fourth Military Medical University

Xi’an, P.R. China

?Work summary: participated in the therapy of liver cancer and have obtained strict training in diagnosis and treatment in tumors and other diseases.

June 1987-May 1990 Graduate student in M.D. program,
Laboratory of Clinic Biochemistry,
Fourth Military Medical University
Xi’an, P.R. China

Research summary: 131-I labeling anti-AFP polyclonal antibody, image analysis and localization in vivo of Hepatoma.

Techniques used: Tissue culture, isotope application, RIA and immunochemistry assays, generation and testing of polyclonal antibodies, immunoelectrophoresis, ELISA, basic surgery techniques for animal experiment.

TEACHING EXPERIENCE

Instructor 1996-1998
Department of Molecular Biology and Biochemistry,
Fourth Military Medical University
Xi’an, P.R. China

GRANTS & AWARDS

1987, Award of excellent teacher for the teaching and editing the textbook.? (In Fourth Military Medical University, China).

1997-1998,Recipient. Grant from Military Sanitation and Science Foundation.

1998-2001, Recipient. Grant from the National fund of Natural Sciences in China.

2005-2008, Recipient. Grant from the National fund of Natural Sciences in China.

PROFESSIONAL MEMBERSHIP

Member, Chinese Society of Biochemistry and Molecular Biology. (1994-)

PUBLICATIONS

• Li J, Zou C, Bai Y [co-first author], et al. Centrobin: a novel daughter centriole protein that is required for centriole duplication, J Cell Biology，2005; 171(3): 437-445.
• Li J, Zou C, Bai Y, et al. DSS1 is required for the stability of BRCA2. Oncogene, 2005;: 1-9.
• Tian XX, Rai D, Li J, Zou C, Bai Y, et al. BRCA2 suppresses cell proliferation via stabilizing MAGE-D1. Cancer Res. 2005; 65(11): 4747-53.
• Zhao JR, Bai YJ, Wang Y, et al. Development of pyrosequencin g approach for rapid screening of rifampin, isoniazid and ethambutol resistances in Mycobacterium tuberculosis. Int J Tuberc Lung Dis, 2005; 9(3):328-32.
• Zhao JR, Bai YJ, Wang Y, et al. Pyrosequencing approach for rapid detection of rifampin resistance of Mycobacterium tuberculosis．Diagn Microbiol Infect Dis, 2005; 51(2): 135-7。
• Wang XF, Gao GD, Yan YB, Zhou J, Wang YW, Su XL, Wang Y, Han FC, Bai YJ, Zhang LF.? Identification of up-regulated genes induced by angiotensin II in cardiac fibroblasts. Acta Physiologica Sinica, 2005; 575: 643-647。(in Chinese)
• Zhao JR, Bai YJ, Wang Y, et al. Rapid detection of rifampin resistance of Mycobacterium tuberculosis using high-throughput pyrosequencing technique. Chin J Tuberc Respir Dis, 2005; 28(5):297-300 (in Chinese)
• Bao JX, Zhao JR, Bai YJ, Zhang LF. Quantification of mRNA expression for angiotensinogen in rat arterical tissues by real-time PCR. Chin J Appl Physiol, 2005; 21(2): 231-4 (in Chinese)
• Xia L, Zhang J, Chen ZC, Bai YJ, et al. Study on relationship between cervical cancer and HLA DQB1*3 and DR15 alleles. J Fourth Mil Med Univ, 2004; 25(24): 2264-6 (in Chinese)
• Xue L, Bai YJ, Zhu WW, et al. A rapid and high throughput detection method of C677T polymorphism in MTHFR gene. J Fourth Mil Med Univ, 2005; 26(14): 1335-7 (in Chinese)
• Zhang WH, Wang Y, Guo HF, Bai YJ, et al. Detection of antidocy against Helicobacter pylori UreB by fluorescence polarized immunoassay using single epitope synthetic peptide as antigen. Chin J Cell Mol Immunol, 2005; 21(3): 371-4 (in Chinese)
• Zhang WH, Bai YJ, Wang Y, et al. A rapid and high throughput method for apoE genotyping. Chin J Med Genet, 2005; 22(1): 61-4 ( in Chinese)
• Zhao JR, Bai YJ, Zhang QH, et al. Detection of hepatitis B virus DNA by real-time PCR using TaqMan-MGB probe technology. World J Gastroentero,. 2005; 11(4): 508-10.
• Bai YJ, Zhao JR, Xue L, et al. A rapid and sensitive new method for detection of TB DNA by fluorescence polarization. Journal of Medical Colleges of PLA, 2004; 19(4): 219-221
• Yang LJ, Bai Yj, Yao LB, et al. Sequencing of light chain variable region antibody against human gastricf cancer and modeling of its three dimensional structure. World Chin J Digestol,? 2004;12(8):1778-1780 (in Chinese)
• Wu GJ, Bai YJ, Wang D, et al. Construction and expression of anti-human CD3 single chain Fv antibody/human P53 tetramerization domain fusion gene. Chin J Cell Mol Immunol,.2004,20(5)：560-562,567.? (in Chinese)
• Wang Y, Zhang WH, Zhao JR, Bai YJ, et al. Detect embB306 gene mutation associated with EMB resistance of M. tuberculosis by TDI-FP technology. Chin J Lab Diagn, 2004,8(5)：455-458 (in Chinese)
• Bai Yj ,Seetha SV,Vamla B, et al. The manufacture and identification of anti-BACP1 antibody. J Fourth Mil Med Univ，2004; 25(1): 30-33. (in Chinese)
• Niu D, Bai YJ, Yan XJ, et al. Correlation between type 2 diabetes mellitus and Pro12Ala polymorphism of peroxisome proliferator-activated receptor gamma (PPAR-γ2) gene. J Fourth Mil Med Univ, 2004;  25(16): 1506－1509 (in Chinese)
• Wu GJ, Wang H, Wang D, Yu L, Hao XK, Bai YJ. Construction and expression of multivalent bispecific single Fv antibody gene specific for human γ-seminoprotien and CD3 molecule. Chin J Urol, 2004, 25(8): 529-532 (in Chinese)
• Chen ZC, Zhang J, Gao YE, Xia L, Bai YJ, Yan XJ. Rapid detection of HPV16 E7 point mutation at codon 29 with fluorescence polarization. J Fourth Mil Med Univ, 2004;  25(15): 1403－1406 (in Chinese)
• Zhang J, Chen ZC, Bai YJ, et al. Cloning and expression of E6 gene of human papillomavirus type 58. J Fourth Mil Med Univ, 2004; 25(3): 201-203? (in Chinese)
• Wang Y, Zhang WH, Zhao JR, Luo M, Zhang ZX, Bai YJ, Yan XJ. Detection KatG 315 gene mutation associated with isoniazid resistance in Mycobacterium tuberculosis by template-directed dye-terminator incorporation with fluorescence polarization detection technology. Chin J Tuberc Respir Dis., 2004; 27(3): 179-182. (in Chinese)
• Guo YH, Lv GT, Bai YJ, et al. Application of PCR-fluorescence polarization for detecting HBV DNA。Laboratory Medicine，2004; 19(2): 128－130 (in Chinese)
• Wu GJ, Wang Z, Wang D, Yu L, Bai YJ. Expression of bispecific single chain Fv antibody gene against human prostate specific antigen and CD3 molecule in Hela cells and its identification of binding affinity. Chin J Exp Surg, 2004; 21(3): 280-281 (in Chinese)
• Guo YH, Zhang J, Zhao JR, Bai YJ, et al. Detecting HBV DNA in serum by PCR-FP. Chin J Lab Med, 2004; 27(1): 26-28 (in Chinese)
• Zhang J, Chen ZC, Bai YJ, et al. Human papillomavirus genotype assay in patients with recurrent condyloma acuminatum by TDI-FP. Chin J Clin Lab Sci, 2004; 22(4): 244-5 (in Chinese)
• Zhang J, Guo YH, Cheng H, Bai YJ, et al. Study of the construction of pLXSN-HPVL2-Angiostatin and its cytolysis capacity on transfected T lymphoma Hut-78 cell in vitro. Chin J Derm Venereol, 2003; 17(6): 366-368 (in Chinese)
• Bai YJ ,Seetha SV,Vamla B,Gao QS。Interaction between BRCA2 and LIM domain only protein FHL2. J Fourth Mil Med Univ, 2003; 24(20): 1845-1848 (in Chinese)
• Bai YJ, Zhao JR, Zhang WH, et al. Detcetion of thiopurine S-methyl -transferase mutations by template directed determinator in incorporation with fluorescence polarization (TDI-FP). Journal of Medical Colleges of PLA, 2003; 18(6): 292-293
• Bai Yu-jie,Niu Dan,Zhao Jin-rong, et al. Rapid detection of PPARr gene Pro12Ala polymorphism with fluorescence polarization in Chinese population. Journal of Medical Colleges of PLA, 2003; 18(3): 197-200
• Bai YJ, Zhao JR, Lv GT, et al. Rapid and high throughput detection of HBV YMDD mutant. World J Gastroenterology (WJG). 2003; 9(10): 2344-2347
• Zhao JR, Bai YJ, Wang SC, et al. Detection of chlamydia trachomatis by real-time polymerase chain reaction using TaqMan-MGB probe technology. Prog Biochem Biophys, 2003; 30(3): 466-450 (in Chinese)
• Lv GT, Lu B, Bai YJ, et al. Paired mutation in core promoter region of HBV in hepatocellular carcinoma by TDI-FP assay. World Chin J Digestol., 2003; 11(7): 916-919 (in Chinese)
• u GJ, Bai YJ, Yu Li, et al. Construction and expression of tetramerized single chain Fv antibody gene specific for human CD3 molecule. Chinese Journal of Biochemistry and Molecular Biology. 2003; 19(5): 594－599 (in Chinese)
• Zhao JR, Bai YJ, Luo M, et al. Detection of Mycobacterium tuberculosis DNA by real-time polymerase chain reaction using TaqMan-MGB probe technology. Chinese Journal of Biochemistry and Molecular Biology,? 2003; 19(6):? 807-810 (in Chinese)
• Zhang J, Yan XJ, Gao YE, Bai YJ. Development of a high throughput assay for human papillomavirus genotypes with fluorescence polarization. China Med J. 2003; 116: 1-5
• Zhang J, Gao YE, Yin GW, Bai YJ, et al. Development of a high throughout gene diagnosis assay for genotyping human papillomavirus. Chin J Lab Med, 2003; 26(3)：145－147 (in Chinese)
• Wu GJ, Hao XK, Bai YJ, et al. Construction and expression of a single chain anti-prostate specific antigen antibody fused to human carboxypeptidase A. J Fourth Mil Med Univ, 2000; 21(4): 391-3
• Bai YJ, Su CZ, Yang AG et al. Analysis of the activity of anti-CD3 ScFv single chain antibody with flow cytometry. J Fourth Mi Med Univ, 1999; 20(7): s17-18
• Bai YJ, Su CZ, Yang AG. Expression, separation and purification of anti-CD3 single-chain antibody. J Cell Mo Immunol,? 1998; 14(3): 201-203
• Bai YJ, Yang AG, Su CZ et al. Construction and sequence analysis of the single-chain Fv gene of an anti-CD3 monoclonal antibody. J Cell Mol Immunol, 1996; 12(3): 6-11
• Bai YJ, Yang AG, Su CZ et al. Purification and Identification of anti-CD3 single-chain antibody. J of Biochem, 1997; 13(s;s): 286-287 (in Chinese).
• Hao XK, Wu GJ, Bai YJ et al.? Construction and expression of the single-chain Fv genes of anti-human game-seminoprotein McAb.? Chin J Urol. 1999; 20(1): 6-8 (in Chinese)
• Wu GJ, Hao XK, Bai YJ. Cloning of human carboxypeptidase A cDNA and its expression in E.coli.? J Cell Mol Immunol, 1999; 15(3):165-168 (in Chinese)
• Wu GJ, Hao XK, Bai YJ et al. Construction and sequence analysis of the single-chain Fv gene of specific for game-seminoprotein.? J Cell Mol Immunol, 1998; 14(3): 101-104
• Bai YJ, Su CZ, Yang AG et al. Analysis of the activity of anti-CD3 ScFv single chain antibody with flow cytometry. ?J Fourth Mil Med Univ, ?1999; 20(7): 17-18 (in Chinese)
• Ji SP, Yao LB, Bai YJ et al. Cloning and expression of genes encoding PH domains of signaling molecules GRF. J Fourth Mil Med Univ, 1999; 20(6): 461-463 (in Chinese)
• Ji SP, Yao LB, Bai YJ et al. Binding of PH domain of signaling molecule PLC -2? to PKC. ??J Cell Mol Immunol. 1999; 15(2): 105-108 (in Chinese)
• Ji SP, Yao LB, Bai YJ et al. Binding of Insulin Receptor Substrate 1 PH Domain to Protein Kinase C. J Biochem Biophys,1999; 31(4): 400-404 (in Chinese)
• Zhang XG, Yan XJ, Bai YJ, et al. Expressin of novel HEK5 gene of tyrosine protein kinase receptor in several human tumor cell lines. J Fourth Mil Med Univ，1998; 19(5): 577-578 (in Chinese)
• Wu GJ, Hao XK, Bai YJ, et al.? Clone and sequence analysis of the light and heavy chain variable region of human monoclonal antibody against game-seminoprotein.? J Fourth Mil Med Univ，1998; 19(5): 484-487 (in Chinese)
• Wu GJ, Hao XK, Bai YJ, et al. Construction and sequence analysis of single chain antibody angainst human game-seminoprotein。J Cell Mol Immunol, 1998; 14(3): 191－194 (in Chinese)
• Wang GH, Chen NC, Hui HX, Nie XY, Bai YJ. Manufacture and activity assay of mouse submaxillary gland nerve growth factor.J Fourth Mil Med Univ,1998; 19(6): 646－648 (in Chinese)
• Zhang XG, Yan XJ, Su CZ, Bai YJ, et al. Amplification, cloning and expression of ecto-gland binding region of Erk in tumor cell lines. Chin J Biochem，1997; 13: 419 (in Chinese)
• Wang F, Wang GH, Niu LG, Bai YJ,et al. Cloning and expression of the HIV binding domain of human CD4.Chin J Biochem，1997; 13: 286－287 (in Chinese)
• Hao XK, Liang GD, Zhang YH, Bai YJ, et al. Radioimmunodetection of prostatic Cancer using 131I labeled anti-seminoprotein mAb. Chin J Medcine, 1993; 73(11): 655-657 (in Chinese)
• Peng ZG, Wang SC, Zhao GN, Bai YJ, Sun BZ. Localization diagnosis of heptatoma by SPECT using I131 labeled anti-AFP antibody。Clin Liver and Gall Dis, 1992; 8(4): 190-191 (in Chinese)
• Bai YJ , Hao XK, Liang GD, et al. Radioimmunodetection of hepatoma:In vivo use 131I? labeled AFP-Ab imaging by single photon emission computed tomography (SPECT). 2nd International Beijing Cancer Symposium 154-155
• Hao XK, Liang GD, Bai YJ, et al. Radioimmunodetection of prostatic Cancer: In vivo use of 131I-(-seminoprotein-Ab for diagnosis of prostatic cancer by nuclear imaging. 2nd International Beijing Cancer Symposium 153-154
• Ji CH, Li BL, Bai YJ. The research of activation of c-myc oncogene in oral squamous-cell epithelioma. J Fourth Mil Med Univ, 1989; 10(4): 282-283 (in Chinese)